Skip to content

Advertisement

You're viewing the new version of our site. Please leave us feedback.

Learn more

BMC Proceedings

Open Access

Rice bran extract (RBE) as supplement for cell culture

  • Satoko Moriyama1,
  • Ken Fukumoto1,
  • Masayuki Taniguchi2,
  • Shigeru Moriyama3,
  • Takuo Tsuno3 and
  • Satoshi Terada1Email author
BMC Proceedings20137(Suppl 6):P106

https://doi.org/10.1186/1753-6561-7-S6-P106

Published: 4 December 2013

Introduction

In mammalian cell culture, fetal bovine serum (FBS) or proteins obtained from mammals are usually supplemented to culture media. Since the use of animal-derived components may cause an infection of virus and other pathogens, alternative supplement derived from non-mammals is eagerly required in cell culture for producing biotherapeutics and for cell therapy [1]. As an alternative supplement, we focused on rice bran extract (RBE), because several studies have been done and reported that RBE has some biological effects such as enhancement of NK cell activity and anti-inflammatory effect on mice [2] and antioxidant effect [3].

Rice bran, by-product of milling in the production of refined white rice, contains abundant nutrients and proteins. In this study, the effect of RBE was examined in the serum-free culture.

Materials and methods

Effect of RBE on several cell lines

RBE was extracted from rice bran in an alkaline solution, precipitated with acid, and subsequently freeze-dried. The proceeding was performed by Tsuno Food Infdustrial Co., Ltd. To test the effect, RBE was supplemented to the culture of hybridoma cells, Chinese hamster ovary cells (CHO-DP12), hepatoma HepG2 and HeLa. The cells were cultured in 24 well plate (Sumitomo Bakelite, Japan) with 1 ml ASF104 medium (Ajinomoto, Japan) containing RBE or BSA (Wako, Japan) as positive control. The cell density was estimated using a hemacytometer. Viable cells were distinguished from dead cells by trypan blue dye exclusion method. The production of antibodies from hybridoma and CHO-DP12 cell was measured by ELISA method.

Fractionation of RBE with UF membrane

In order to identify the growth factor(s) in RBE, fractionations were performed using UF membranes. RBE was fractionated into the permeable and residual fraction with ultrafiltration membrane Amicon Ultra-15 (Merck Millipore, Germany) at 4,000 rpm, 40 min and 4°C. The fractions and whole RBE were added to the culture of hybridoma and CHO-DP12 cells.

Results and discussion

Enhanced cell growth and productivity using RBE

Figure 1 shows an enhanced proliferation by RBE. On growth and monoclonal antibody production of hybridoma cells, RBE had desired effect and the effect of RBE was superior to that of BSA. Similarly, to CHO-DP12 cells, addition of RBE exhibited increased cell growth and improved the productivity of humanized antibody. Growth of HepG2 and HeLa cells were also enhanced in the presence of RBE.
Figure 1

Cell growth in serum-free medium containing RBE. a mouse hybridoma cell, b HeLa.

Improvement of fractionated RBE by UF membrane

Fractionated RBEs by UF membranes were also tested. The fraction of RBE more than 60 kDa improved the proliferation of hybridoma cells and the level was superior to that of whole RBE, while the fraction less than 60 kDa inhibited the proliferation. This results suggest that in RBE, some lower molecular inhibitor(s) and higher molecular growth factor(s) would be contained.

Conclusion

We provide the first evidence that RBE is an attractive culture supplement to improve the proliferation and the production of mammalian cells.

Authors’ Affiliations

(1)
University of Fukui
(2)
Niigata University
(3)
Tsuno Food Industrial Co., Ltd

References

  1. Leopold G, Thomas RK, Sonia N, Manfred R: Emerging trends in plasma-free manufacturing of recombinant protein therapeutics expressed in mammalian cells. Biotechnology journal. 2009, 4: 186-201.View ArticleGoogle Scholar
  2. Kim HY, Kim JH, Yang SB, Hong SG, Lee SA, Hwang SJ, Shin KS, Suh HJ, Park MH: A polysaccharide extracted from rice bran fermented with Lentinus edodes enhances natural killer cell activity and exhibits anticancer effects. Journal of medicinal food. 2007, 10: 25-31.View ArticlePubMedGoogle Scholar
  3. Elisa R, Consuelo SM, Miramontes E, Juan B, Ana GM, Olga C, Rosa C, Juan P: Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of water-soluble enzymatic extract from rice bran. Food Research International. 2009, 42: 387-393.View ArticleGoogle Scholar

Copyright

© Moriyama et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement