Size-dependent antioxidative activity of platinum nanoparticles
© Nakanishi et al.; licensee BioMed Central Ltd. 2013
Published: 4 December 2013
So far, most of studies on nanometer-sized metal particles have focused on biological safety and potential hazards. However, anti-oxidative activity of noble metal nanoparticles (NPs) attracts much attention, recently. Platinum nanoparticles (Pt NPs) are one of the most important noble metals in nanotechnology because Pt NPs have negative surface potential from negative charges and are stably suspended from an electric repulsion between the same charged particles . We previously reported that Pt NPs of 2-3 nm sizes scavenged reactive oxygen species (ROS) such as superoxide anion radical, hydrogen peroxide and hydroxyl radical in vitro . Here, we report the cytotoxicity and size-dependent antioxidative activity of Pt NPs on rat skeletal muscle cell line, L6.
Materials and methods
Pt NPs were synthesized by a modified citrate reduction method of Hydrogen hexachloroplatinate (IV). Particle size and concentrations of Pt NPs were determined by a transmittance electron microscope (TEM) and ICP-MS, respectively. To find the toxic effect of Pt NPs rat myoblast L6 cells were pre-cultured for 24 hours in culture medium with a 10-3 to 10 mg/l of Pt NPs and cell viability was determined by WST-1 assay. To investigate the anti-oxidative effect of Pt NPs on L6 cells, the relative amount of intracellular H2O2 was measured with a Bes-H2O2-AC florescent probe, which is designed to detect intracellular H2O2 specifically . The intracellular ROS levels when treated with 1 mg/l of Pt NPs for 2 hours were measured using IN Cell Analyzer 1000.
Results and conclusions
Our results suggest Pt NPs of 2-3 nm sizes have no cytotoxity below 10 mg/l and are useful materials to scavenge ROS. In this regard Pt NPs are expected as redox regulation factors for suppression of various ROS-related diseases.
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