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Feeding strategy optimization in interaction with target seeding density of a fed-batch process for monoclonal antibody production
© Clincke et al.; licensee BioMed Central Ltd. 2013
Published: 4 December 2013
Current trend towards Quality by Design (QbD) leads the process development exercise towards systematic experimentation, rational development, process understanding, characterization and control. In this study, an example of the application of QbD approach is given. Optimization of the feeding strategy and the target seeding density was performed and interactions of the two parameters were assessed in order to enhance cell growth and MAb productivity. The feeding strategy was optimized to take into account daily process performance attributes and associated nutrient needs of the culture to maintain a balance between metabolism and MAb productivity. For scale up the feed strategy was simplified to become independent of daily process performance attributes. Feed ranging studies were performed to assess the robustness of the process.
Materials and methods
2L stirred tank bioreactors were run for 14 days in a fed-batch mode in a chemically defined medium. Feed was added daily from day 3 onwards. If required, antifoam C was added to the bioreactor by manual injections. DO, pH, and temperature were controlled at setpoint. DO was controlled using a multi-stage aeration cascade via a ring sparger. Viable cell concentration, cell viability, and average cell diameter were measured using a ViCell cell counter. The glucose, lactate, glutamine and ammonia concentrations were measured with a BioProfile Analyzer 400. On the day of harvest, the clarification was performed by centrifugation plus depth filtration. Monoclonal Antibody (MAb) concentration of the supernatant samples was quantified using Octet QK and Protein A high performance liquid chromatography.
Interaction study between feeding strategy and Target Seeding Density (TSD)
MAb titers and Product Quality Attributes observed during the feed ranging study
MAb titer (Normalized)
Mannose 5 (Normalized)
Center point (n = 2)
+20% Feed (n = 2)
-20°% Feed (n = 2)
Feeding strategy simplification, mode of feed addition, feeding ranging study
The design of the feeding strategy was simplified in order to facilitate the process transfer to large scale manufacture. Hence, based on the final feed ratio, the feed rates were fixed with a feed volume independent of the projected subset of process performance attributes. The pH of the feed is highly basic. In our 2L experiments, feed was added within less than 5 min, which generates pH excursions above 7.40. A strategy of slow bolus addition with a fixed minimum addition timeframe and with a fixed maximum flow rate was implemented, leading to minor pH-excursions during feeding with only minor CO2 flows necessary to keep the pH within the pH deadband (data not shown). The robustness of the process was assessed by performing an experiment with over- and underfeeding cultures. Underfeeding at 20% below target had no impact on process performance (MAb titer) while feeding 20% above target led to a lower MAb titer (Table 1). No impact of underfeeding or overfeeding at ± 20% of the feed target was observed on the Acidic Peak Group (APG) and aggregate levels. Feeding 20% above target led to an increase in Mannose 5 species.
DoE enabled us to study the impact of the feed addition strategy and the impact of the TSD on the Mab titer and PQAs at harvest in a time efficient manner. The feeding strategy was simplified to become independent of the projected subset of process performance attributes and to be scalable to large scale manufacture. The mode of feed addition was optimized to minimize pH-excursions during feeding. Feed ranging studies showed that underfeeding at 20% below target had no impact on MAb titer and PQAs while feeding 20% above target led to a lower MAb titer and an increase in Mannose 5 species (glycan). Finally, a 36% increase in the MAb titer was achieved in the feed optimized conditions compared to control condition at harvest with a feed strategy designed to be robust and scalable.
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