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BMC Proceedings

Open Access

Evaluation of antifungal and cytotoxic activity of trans-Chalcone and α-Solanine

  • Tatiana Takahasi Komoto1,
  • Gabriel Silva1,
  • Tamires Bitencourt1,
  • Bruna Azevedo Cestari1,
  • Mozart Marins1 and
  • Ana Lúcia Fachin1
BMC Proceedings20148(Suppl 4):P36

https://doi.org/10.1186/1753-6561-8-S4-P36

Published: 1 October 2014

Background

Dermatophytes are adapted to grow in keratinized tissues such as skin, nail and hair. Trichophyton rubrum is the most frequent cause of dermatophytosis in Brazil and in the world [1]. Despite its incidence there are only a limited number of antifungal drugs available for clinical use and some drugs are highly toxic to humans. In this regard, chalcones and alkaloids are phytochemical products which provide a rich source of chemical diversity for the development of new antifungals. Chalcones inhibit the biosynthesis of the cell wall and activity of fatty acid synthase in yeast [2, 3]. The glycoalkaloid α-Solanine purified from potate sprout presents antifungal activity by altering cell membrane integrity and inhibition of sporulation [4]. The aim of the present study was to evaluate the minimum inhibitory concentration (MIC) and cytotoxicity (by MTT) of trans-Chalcone and α-Solanine toward strain MYA3108 of T.rubrum and the keratinocyte cell line HaCat, in order to evaluate the potential use of these phytochemicals against fungal skin infection.

Materials and methods

The antifungal activity of the compounds was determinated by using the M38-A microdilution technique according to the Clinical and Laboratory Standards Institute [5] toward strain T.rubrum for 7 days at 28°C. Keratinocytes were cultures in RPPMI supplemented with 10% fetal calf serum and incubated at 37°C and 5% CO2. Cells were plated (2.5x105 cells/mL) in a 96-well tray 24 h prior to the beginning of the experiment. After addition of several concentrations of natural compounds or the vehicle, cells were analyzed after a period of 24 h using the MTT assay.

Results

The MICs of α-Solanine and trans-Chalcone were 7.8 µg/mL, showing effectiveness against T. rubrum, while the inhibition of the HaCat cell line by trans-Chalcone (7.8 µg/ml) and α-Solanine (50 µg/ml) were 45.78% and 68.86% respectively.

Conclusions

Finally, the α-Solanine is a potential candidate for the development of new antifungal drugs against T. rubrum, due to its significant antifungal activity and lower citotoxicity for human keratinocytes.

Financial support

This work was supported for CAPES and FAPESP.

Authors’ Affiliations

(1)
Universidade de Ribeirão Preto

References

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Copyright

© Komoto et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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