Background
Chinese Hamster Ovary (CHO) derived cells are the most commonly used cell lines for the production of biopharmaceuticals. We developed a plug and play method to optimize the growth medium for a given CHO production cell line. The experiment consists of a Mixture Design of Experiments (DOE) approach using different basal media to select the optimized formulations for a specific CHO cell line. The package for media optimization consists of different base media together with the ambr15 high throughput bioreactor and integrated MODDE DoE software.