Media optimization for CHO fed-batch processes using a DoE approach in automated high-throughput single use ambr15 bioreactors
© Grimm et al. 2015
Published: 14 December 2015
Chinese Hamster Ovary (CHO) derived cells are the most commonly used cell lines for the production of biopharmaceuticals. We developed a plug and play method to optimize the growth medium for a given CHO production cell line. The experiment consists of a Mixture Design of Experiments (DOE) approach using different basal media to select the optimized formulations for a specific CHO cell line. The package for media optimization consists of different base media together with the ambr15 high throughput bioreactor and integrated MODDE DoE software.
Methods and results
The ambr15 and MODDE software used here provided a path to obtain effective media formulations for a given specific CHO cell line. The approach consists of a comprehensive service containing four basal media to prepare the mixes and the ambr15. It is supported by an application specialist to guide the experiments and interpretation of results.
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