Volume 9 Supplement 9

24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products

Meeting abstracts

Edited by Francesc Gòdia

Publication charges for this supplement were funded by the European Society for Animal Cell Techonology (ESACT). The Supplement Editor declares that he has no competing interests.

24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products.

Barcelona, Spain31 May - 3 June 2015

Concatenated PDF 9s9

Page 1 of 2

Content type: Oral presentation

Front-line of genome editing technology for animal cell engineering

Authors: Tetsushi Sakuma

Citation: BMC Proceedings 2015 9(Suppl 9):O1

Published on: 14 December 2015
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Content type: Oral presentation

Human pluripotent stem cell process parameter optimization in a small scale suspension bioreactor

Authors: Yonatan Y Lipsitz and Peter W Zandstra

Citation: BMC Proceedings 2015 9(Suppl 9):O10

Published on: 14 December 2015
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Content type: Oral presentation

Protein aerosol for intranasal nose to brain (N2B) delivery

Authors: Martina Stützle, Stefan Carle, Lucas Engelhardt, Ulrich Simon, Annette Schafmeister, Chrystelle Mavoungou and Katharina Schindowski

Citation: BMC Proceedings 2015 9(Suppl 9):O11

Published on: 14 December 2015
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Content type: Oral presentation

Development of a mammalian cell culture process for rapid Clinical-Scale production of novel Influenza Nanoparticle vaccines

Authors: Payal Biswas, Christian Trozado, James Lee and Richard M Schwartz

Citation: BMC Proceedings 2015 9(Suppl 9):O12

Published on: 14 December 2015
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Content type: Oral presentation

Purification of modified vaccinia virus Ankara from suspension cell culture

Authors: Ingo Jordan, Diana Weimer, Stefan Iarusso, Holger Bernhardt, Verena Lohr and Volker Sandig

Citation: BMC Proceedings 2015 9(Suppl 9):O13

Published on: 14 December 2015
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Content type: Oral presentation

Adventitious agent contamination risk mitigation: engineering MMV virus resistance into CHO cells

Authors: Joaquina X Mascarenhas, Lisa Burger, Ademola Kassim, Trissa Borgschulte, Delia Lyons, Henry George, Nan Lin, Audrey Chang, David Onions, David Pintel and Kevin Kayser

Citation: BMC Proceedings 2015 9(Suppl 9):O2

Published on: 14 December 2015
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Content type: Oral presentation

Production of IgGs with a human-like sialylation in CHO cells

Authors: Céline Raymond, Anna Robotham, Maureen Spearman, Michael Butler, John Kelly and Yves Durocher

Citation: BMC Proceedings 2015 9(Suppl 9):O3

Published on: 14 December 2015
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Content type: Oral presentation

Identification of process parameters influencing product quality in mammalian cell culture

Authors: Albert J Paul and Friedemann Hesse

Citation: BMC Proceedings 2015 9(Suppl 9):O4

Published on: 14 December 2015
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Content type: Oral presentation

Non-fucose level as a function of glycoenzyme transcription in a glycoengineered CHO cell line

Authors: Sven Markert, Harald Wizemann and Christine Jung

Citation: BMC Proceedings 2015 9(Suppl 9):O5

Published on: 14 December 2015
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Content type: Oral presentation

Understanding the impact of different bioprocess conditions on monoclonal antibody glycosylation in CHO cell cultures through experimental and computational analyses

Authors: Si N Sou, Christopher Sellick, Ken Lee, Alison Mason, Sarantos Kyriakopoulos, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2015 9(Suppl 9):O6

Published on: 14 December 2015
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Content type: Oral presentation

Process parameters impacting product quality

Authors: Jan Bechmann, Frederik Rudolph, Lucy Gebert, Jochen Schaub, Benedikt Greulich, Michael Dieterle and Harald Bradl

Citation: BMC Proceedings 2015 9(Suppl 9):O7

Published on: 14 December 2015
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Content type: Oral presentation

Multi-omics approach for comparative studies of monoclonal antibody producing CHO cells

Authors: Camila A Orellana, Esteban Marcellin, Trent Munro, Peter P Gary and Lars K Nielsen

Citation: BMC Proceedings 2015 9(Suppl 9):O8

Published on: 14 December 2015
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Content type: Oral presentation

Development of an advanced cell therapy product indicated for the treatment of gonarthrosis

Authors: Joaquim Vives, Margarita Blanco, Marta Caminal, Maria I Coca, Margarita Codinach, Ruth Coll, Manuel Doral, Mireia Lloret, Irene Oliver-Vila, Isabel Ortega, Laura Reales, Míriam Requena-Montero, Luciano Rodríguez, Sílvia Torrents and Joan García

Citation: BMC Proceedings 2015 9(Suppl 9):O9

Published on: 14 December 2015
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Content type: Poster presentation

Stability difference of each chromosome in Chinese Hamster Ovary cell line

Authors: Noriko Yamano, Toshitaka Kumamoto, Mai Takahashi, Jana Frank, Masayoshi Onitsuka and Takeshi Omasa

Citation: BMC Proceedings 2015 9(Suppl 9):P1

Published on: 14 December 2015
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Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster Ovary cells

Authors: Christine L Chin, Hing K Chin, Cara S Hui Chin, Ethan T Lai and Say KongNg

Citation: BMC Proceedings 2015 9(Suppl 9):P10

Published on: 14 December 2015
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Content type: Poster presentation

Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion

Authors: YuanShan Lai, Noriko Yamano, Masayoshi Onitsuka and Takeshi Omasa

Citation: BMC Proceedings 2015 9(Suppl 9):P11

Published on: 14 December 2015
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Expression of glycoproteins with excellent glycosylation profile and serum half-life in CAP-Go cells

Authors: Silke Wissing, Jens Wölfel, Helmut Kewes, Christian Niehus, Corinna Bialek, Sabine Hertel and Nicole Faust

Citation: BMC Proceedings 2015 9(Suppl 9):P12

Published on: 14 December 2015
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Content type: Poster presentation

Generation and characterization of a superior host cell line for biomanufacturing

Authors: Anett Ritter, Johannes Wienberg, Bernd Voedisch, Burkhard Wilms, Sabine Geisse, Thomas Jostock and Holger Laux

Citation: BMC Proceedings 2015 9(Suppl 9):P13

Published on: 14 December 2015
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Content type: Poster presentation

Novel cell engineering of the Unfolded Protein Response to achieve efficient therapeutic protein production cell line

Authors: Michael C Song, Jeff J Hou, Lars K Nielsen and Peter P Gray

Citation: BMC Proceedings 2015 9(Suppl 9):P14

Published on: 14 December 2015
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Content type: Poster presentation

Media optimization for CHO fed-batch processes using a DoE approach in automated high-throughput single use ambr15 bioreactors

Authors: Christophe Grimm, Wolfgang Kusser, Brian Lee, Greg Bremer and Alexis Bossie

Citation: BMC Proceedings 2015 9(Suppl 9):P15

Published on: 14 December 2015
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Content type: Poster presentation

Cell Line Generation: Relying on tricks or tools of the trade?

Authors: Jeff JC Hou, Eric Zhu, Michael Song, Kar M Leung, Martina Jones, Trent P Munro and Peter P Gray

Citation: BMC Proceedings 2015 9(Suppl 9):P16

Published on: 14 December 2015
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Content type: Poster presentation

Technology toolbox for cell line development - next generation cell line development technologies

Authors: Holger Laux, Ursula Bodendorf, Sandrine Romand, Anett Ritter, Thomas Jostock and Burkhard Wilms

Citation: BMC Proceedings 2015 9(Suppl 9):P17

Published on: 14 December 2015
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Content type: Poster presentation

Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization

Authors: Sebastian Püngel, Miklos Veiczi, Tim F Beckmann, Vanessa Vater, Penélope V Soto, Renée Ermerling, Derek Levison, Christoph Heinrich and Tim Welsink

Citation: BMC Proceedings 2015 9(Suppl 9):P18

Published on: 14 December 2015
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Content type: Poster presentation

Construction of a gene knockout CHO cell line using a simple gene targeting method

Authors: Marina Aga, Noriko Yamano, Toshitaka Kumamoto, Jana Frank, Masayoshi Onitsuka and Takeshi Omasa

Citation: BMC Proceedings 2015 9(Suppl 9):P2

Published on: 14 December 2015
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Integrating skin and vasculature in a Multi-Organ-Chip Platform

Authors: Katharina Schimek, Annina Markhoff, Frank Sonntag, Martin Blechert, Roland Lauster, Uwe Marx and Gerd Lindner

Citation: BMC Proceedings 2015 9(Suppl 9):P20

Published on: 14 December 2015
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Content type: Poster presentation

Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

Authors: Nadine Kochanowski, Gaetan Siriez, Larissa Mukankurayija, Aurélie Delangle, Alex Murray-Smith, Kevin Dromer, David Mainwaring, Clemens Stilling, Nadja Prang, Laetitia Malphettes and Annick Gervais

Citation: BMC Proceedings 2015 9(Suppl 9):P21

Published on: 14 December 2015
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Content type: Poster presentation

Protection of bioreactor culture from virus contamination by use of a virus barrier filter

Authors: Kimberly Mann, Jon Royce, Christina Carbrello, Robert Smith, Rong-Rong Zhu, Yuanchun Zeng, Anne Leahy, Mary Priest, Ven Raman, Jayant S Nick Harrington, Scott Bliss, William Bryant, Nhung Nguyen, Soleil Le, Danielle DeCesaro, Jeremy Perreault…

Citation: BMC Proceedings 2015 9(Suppl 9):P22

Published on: 14 December 2015
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Optimization and scale-up of cell culture and purification processes for production of an adenovirus-based tuberculosis vaccine

Authors: Chun F Shen, Danielle Jacob, Zhongqi Shao, Alice Bernier, Xuefeng Yu, Mehul Patel, Tao Zhu and Amine Kamen

Citation: BMC Proceedings 2015 9(Suppl 9):P23

Published on: 14 December 2015
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Content type: Poster presentation

Antibody disulfide bond reduction during process development: Insights using a scale-down model process

Authors: Julie Ruaudel, Martin Bertschinger, Sonia Letestu, Roberto Giovannini, Paul Wassmann and Pierre Moretti

Citation: BMC Proceedings 2015 9(Suppl 9):P24

Published on: 14 December 2015
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Content type: Poster presentation

Novel small scale TFF cell retention device for perfusion cell culture systems

Authors: Chris S Martin, Jorge Padilla-Zamudio, Doug Rank, Patrick McInnis, Mikhail Kozlov, Sarah Reynolds, Joe Parella and Lee Madrid

Citation: BMC Proceedings 2015 9(Suppl 9):P25

Published on: 14 December 2015
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Content type: Poster presentation

A novel low-parameter computational model to aid in-silico glycoengineering

Authors: Philipp N Spahn, Anders H Hansen, Henning G Hansen, Johnny Arnsdorf, Helene F Kildegaard and Nathan E Lewis

Citation: BMC Proceedings 2015 9(Suppl 9):P26

Published on: 14 December 2015
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Content type: Poster presentation

Medium development beyond production media: Chemically defined media for transfection and single cell cultivation

Authors: Tim F Beckmann, Sandra Klausing, Sebastian Püngel, Juliana Coronel, Tim Welsink and Christoph Heinrich

Citation: BMC Proceedings 2015 9(Suppl 9):P27

Published on: 14 December 2015
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Content type: Poster presentation

An improved model framework linking the extracellular environment to antibody glycosylation

Authors: Philip M Jedrzejewski, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2015 9(Suppl 9):P28

Published on: 14 December 2015
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Content type: Poster presentation

Quality compliance in the development of cell-based medicines in non-pharma environments

Authors: Irene Oliver-Vila, Amy L van Deusen, Roger Palau and Joaquim Vives

Citation: BMC Proceedings 2015 9(Suppl 9):P29

Published on: 14 December 2015
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Content type: Poster presentation

Establishment and characterization of new human cell lines for recombinant therapeutic protein production

Authors: Yuichi Inoue, Akira Iwamoto, Aiko Inoue and Hiroharu Kawahara

Citation: BMC Proceedings 2015 9(Suppl 9):P3

Published on: 14 December 2015
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A novel measuring chamber and automation platform for mammalian cell culture processes

Authors: Tim H Lücking, Christoph Busse, Christian Lüder, David Bulnes-Abundis, Dörte Solle and Thomas Scheper

Citation: BMC Proceedings 2015 9(Suppl 9):P30

Published on: 14 December 2015
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Content type: Poster presentation

Spectroscopic tools for an automated suspension cell culture screening system

Authors: Carsten Musmann, Klaus Joeris and Sven Markert

Citation: BMC Proceedings 2015 9(Suppl 9):P31

Published on: 14 December 2015
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Content type: Poster presentation

Monitorization of pH and OTR using a multiple shake flask platform: A tool for metabolism and cell growth assessment in mammalian cell cultures

Authors: Leticia Liste-Calleja, Tibor Anderlei, Jonatan López-Repullo, Adrián Urbano, Martí Lecina and Jordi J Cairó

Citation: BMC Proceedings 2015 9(Suppl 9):P32

Published on: 14 December 2015
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Content type: Oral presentation

Identification of performance drivers for an antibody producing CHO-S cell line culture in the Allegro™ XRS 20 single-use bioreactor utilizing historical data

Authors: Todd Sanderson, Alex Martino, Ing-Kae Wang, Amanda Rose, Byron Rees and Lisa Bradbury

Citation: BMC Proceedings 2015 9(Suppl 9):P33

Published on: 14 December 2015
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From Clone Selection to 80L Bioreactor Production: Better, Faster, Leaner

Authors: Marie-Françoise Clincke, Frédéric Delouvroy, Guillaume L Révérend, Jimmy Stofferis, Matthew Hinchliffe and Laetitia Malphettes

Citation: BMC Proceedings 2015 9(Suppl 9):P34

Published on: 14 December 2015
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Overcoming the clarification challenges of high cell density culture

Authors: Laura Gimenez, Elie E Kawkabani, Pieter Jacobs and Laetitia Malphettes

Citation: BMC Proceedings 2015 9(Suppl 9):P35

Published on: 14 December 2015
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Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44

Authors: David Reinhart, Lukas Damjanovic, Wolfgang Sommeregger, Andreas Gili, Stanislaus Schafellner, Andreas Castan, Christian Kaisermayer and Renate Kunert

Citation: BMC Proceedings 2015 9(Suppl 9):P36

Published on: 14 December 2015
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Method development and Application of Growth Phase specific recombinant Protein Glycosylation Analysis

Authors: Klaudia Grunwald, Thomas Noll and Heino Büntemeyer

Citation: BMC Proceedings 2015 9(Suppl 9):P37

Published on: 14 December 2015
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The potential of small molecules to modulate glycosylation by media design

Authors: David Brühlmann, Anaïs Muhr, Jürgen Hemberger, Markus Sauer, Henri Kornmann, Martin Jordan and Hervé Broly

Citation: BMC Proceedings 2015 9(Suppl 9):P38

Published on: 14 December 2015
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Technological and methodical process integration in manufacturing of monoclonal antibodies

Authors: Petra Gronemeyer and Jochen Strube

Citation: BMC Proceedings 2015 9(Suppl 9):P39

Published on: 14 December 2015
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Use of a new genetic element to improve expression level of cell lines for recombinant protein production

Authors: Emilie Vaxelaire, Lauriane Gamand, Christel Aebischer-Gumy, Pierre Moretti and Martin Bertschinger

Citation: BMC Proceedings 2015 9(Suppl 9):P4

Published on: 14 December 2015
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High-titer expression of recombinant antibodies by transiently transfected HEK 293-6E cell cultures

Authors: Volker Jäger, Johanna Groenewold, Dominik Krüger, Dennis Schwarz and Veith Vollmer

Citation: BMC Proceedings 2015 9(Suppl 9):P40

Published on: 14 December 2015
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Revisiting the determination of hydromechanical stresses encountered by microcarriers in stem cell culture bioreactors

Authors: Angélique Delafosse, Marie-Laure Collignon, Annie Marc, Dominique Toye and Eric Olmos

Citation: BMC Proceedings 2015 9(Suppl 9):P41

Published on: 14 December 2015
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Model-based DoE for feed batch cultivation of a CHO cell line

Authors: Johannes Möllerl, Regine Eibl, Dieter Eibl and Ralf Pörtner

Citation: BMC Proceedings 2015 9(Suppl 9):P42

Published on: 14 December 2015
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Considerations for cell passaging in cell culture seed trains

Authors: Tanja H Rodríguez, Ralf Pörtner and Björn Frahm

Citation: BMC Proceedings 2015 9(Suppl 9):P43

Published on: 14 December 2015
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24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products

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