Chinese hamster ovary (CHO) cells have become the preferred expression system for the production of complex recombinant proteins. In this study, chemically defined CHO cell culture media and feed substrates from different vendors were investigated regarding their influence on cell metabolism, antibody titer and quality. Special emphasis was put on elucidating how these attributes change with the use of different CHO host cell lines. For this purpose, CHO-K1, CHO-DG44, and CHO-S - each producing the same IgG antibody - were adapted to ActiCHO™ P and CD CHO medium. All three cell lines were grown both in batch and in fed-batch cultures using the manufacturer's specific concentrated feed supplements. The impact of the different media and feeds on antibody production, cell growth, cell-specific nutrient consumption, by-product formation and IgG quality was analyzed throughout the process.