Decision tree for selection of suitable cultivation parameters for mammalian cell culture processes
© Pörtner et al. 2015
Published: 14 December 2015
Development of bioprocesses for mammalian cells has to deal with different bioreactor types and scales. Bio-reactors might be intended for seed train and production, research, process development, validation or transfer purposes. During these activities, not only the problem of up- and downscaling might lead to failure of repro-ducibility, but also the use of different bioreactor geometries and operation conditions. In such cases, the criteria for bioreactor design and process transfer should be re-evaluated in order to avoid an erroneous transfer of cultivation parameters.
For selection of process conditions several questions can be asked:
Type and scale of the intended cultivation system
Which data are required (cell specific parameters, specific data for the cultivation system)?
Are appropriate data e.g. for cell growth, substrate uptake, medium composition available?
For which cultivation systems have these data been determined?
Are data on power input, mixing time, oxygen transfer etc. available?
Which methods can be used to determine or estimate the above mentioned parameters?
For selection and evaluation of suitable cultivation parameters a decision tree (Figure 1) has been formulated to provide a guideline for design of mammalian cell culture processes. References for process transfer strategies are given for the following cases:
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