22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Analysis of the mitochondrial subproteome of the human cell line AGE1.HN – a contribution to a systems biology approach

Authors: Eva Schräder, Sebastian Scholz, Volker Sandig, Raimund Hoffrogge and Thomas Noll

Characterisation of cultivation of the human cell line AGE1.HN.AAT

Authors: Eva Schräder, Sebastian Scholz, Jens Niklas, Alexander Rath, Oscar Platas Barradas, Uwe Jandt, Volker Sandig, Thomas Rose, Ralf Pörtner, Udo Reichl, An-Ping Zeng, Elmar Heinzle and Thomas Noll

Strategies in umbilical cord-derived mesenchymal stem cells expansion: influence of oxygen, culture medium and cell separation

Authors: Antonina Lavrentieva, Tim Hatlapatka, Ramona Winkler, Ralf Hass and Cornelia Kasper

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Discerning key parameters influencing high productivity and quality through recognition of patterns in process data

Authors: Huong Le, Marlene Castro-Melchor, Christian Hakemeyer, Christine Jung, Berthold Szperalski, George Karypis and Wei-Shou Hu

Proteomic and metabolomic characterization of CHO DP-12 cell lines with different high passage histories

Authors: Tim Beckmann, Tobias Thüte, Christoph Heinrich, Heino Büntemeyer and Thomas Noll

A method for metabolomic sampling of suspended animal cells using fast filtration

Authors: Martin Volmer, Julia Gettmann, Sebastian Scholz, Heino Büntemeyer and Thomas Noll

Analysis of glycolytic flux as a rapid screen to identify low lactate producing CHO cell lines with desirable monoclonal antibody yield and glycan profile

Authors: Rachel Legmann, Julie Melito, Ilana Belzer and David Ferrick

Influence of cell specific productivity on product quality

Authors: Ruchika Srivastava, Lavanya Rao, Kriti Shukla, Sunaina Prabhu, Saravanan Desan, Dinesh Baskar, Ankur Bhatnagar, Anuj Goel and Harish Iyer

A novel peptide to enhance recombinant BMP-2 production in mammalian cell cultures

Authors: Aileen J Zhou, Cameron ML Clokie and Sean AF Peel

Determination of intracellular antibody production, cell density, and viability of recombinant CHO-DG44 cells using the MACSQuant® Analyzer

Authors: Jörn Pluschke, Sandra Klausing, Annika Haseloff, Bernd Schröder and Tanno Hübel

Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays

Authors: Sandra Klausing, Oliver Krämer and Thomas Noll

Characterization of chromatographic yeast extract fractions promoting CHO cell growth

Authors: Mathilde Mosser, Romain Kapel, Arnaud Aymes, Laurent-Michel Bonanno, Eric Olmos, Iris Besançon, Dominique Druaux, Isabelle Chevalot, Ivan Marc and Annie Marc

Study of expansion of porcine bone marrow mesenchymal stem cells on microcarriers using various operating conditions

Authors: Caroline Ferrari, Frédérique Balandras, Emmanuel Guedon, Eric Olmos, Nguyen Tran, Isabelle Chevalot and Annie Marc

Growth and death kinetics of CHO cells cultivated in continuous bioreactor at various agitation rates

Authors: Frédérique Balandras, Eric Olmos, Caroline Hecklau, Fabrice Blanchard, Emmanuel Guedon and Annie Marc

Increasing antibody yield and modulating final product quality using the Freedom^TM CHO-S^TM production platform

Authors: Michelle Sabourin, Ying Huang, Prasad Dhulipala, Shannon Beatty, Jian Liu, Peter Slade, Shawn Barrett, Shue-Yuan Wang, Karsten Winkler, Susanne Seitz, Thomas Rose, Volker Sandig, Peggy Lio, Steve Gorfien, Laurie Donahue-Hjelle and Graziella Piras

Single use bioreactors for the clinical production of monoclonal antibodies – a study to analyze the performance of a CHO cell line and the quality of the produced monoclonal antibody

Authors: Sonja Diekmann, Constanze Dürr, Alexander Herrmann, Ingo Lindner and Daniela Jozic

Development of live cultural pandemic influenza vaccine Vector-Flu

Authors: Elena A Nechaeva, Tatyana Y Sen’kina, Alexander B Ryzhikov, Irina F Radaeva, Ol’ga G P’yankova, Natal’ya V Danil’chenko, Tatyana M Sviridenko, Marina P Bogryantzeva, Natal’ya V Gilina, Nikolay A Varaksin, Tatyana G Ryabicheva, Irina V Kiseleva and Larisa G Rudenko

Study of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE Bioreactor™

Authors: Marie-Françoise Clincke, Carin Mölleryd, Ye Zhang, Eva Lindskog, Kieron Walsh and Véronique Chotteau

Utilizing Roche Cedex Bio analyzer for in process monitoring in biotech production

Authors: Dörthe Druhmann, Sabrina Reinhard, Felizitas Schwarz, Christina Schaaf, Katrin Greisl and Tim Nötzel

LDH-C can be differentially expressed during fermentation of CHO cells

Expression of CHO mRNA was measured with special microarrays from the Consortium for Chinese Hamster Ovary (CHO) Cell Genomics led by Prof. Wei-Shou Hu of the University of Minnesota and Prof. Miranda Yap of t...

Authors: Berthold Szperalski, Christine Jung, Zhixin Shao, Anne Kantardjieff and Wei-Shou Hu

Stem cell: from basic theoretical assumptions and mathematical concepts to the computational models

Authors: Katya Simeonova and Ganka Milanova

Comparison of the activity and pluripotency maintaining potential of human leukemia inhibitory factor (LIF) produced in E.coliand CHO cells

Authors: Claas Haake, Sophia Bonk, Jana Parsiegla, Magda Tomala, Komal Loya, Malte Sgodda, Tobias Cantz, Axel Schambach, Cornelia Kasper and Thomas Scheper

Disulphide bond reduction of a therapeutic monoclonal antibody during cell culture manufacturing operations

Authors: Brian Mullan, Bryan Dravis, Amareth Lim, Ambrose Clarke, Susan Janes, Pete Lambooy, Don Olson, Tomas O’Riordan, Bruce Ricart and Alexander G Tulloch

Preliminary evaluation of microcarrier culture for growth and monoclonal antibody production of CHO-K1 cells

Authors: M Elisa Rodrigues, Pedro Fernandes, A Rita Costa, Mariana Henriques, Joana Azeredo and Rosário Oliveira

Strategies for adaptation of mAb-producing CHO cells to serum-free medium

Authors: A Rita Costa, M Elisa Rodrigues, Mariana Henriques, Rosário Oliveira and Joana Azeredo

Impact of different influenza cultivation conditions on HA N-Glycosylation

Authors: Jana V Roedig, Erdmann Rapp, Yvonne Genzel and Udo Reichl

Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes

Authors: Marie-Françoise Clincke, Emmanuel Guedon, Frances T Yen, Virginie Ogier and Jean-Louis Goergen

Characterization of metalloprotease and serine protease activities in batch CHO cell cultures: control of human recombinant IFN-γ proteolysis by addition of iron citrate

Authors: Marie-Françoise Clincke, Emmanuel Guedon, Frances T Yen, Virginie Ogier and Jean-Louis Goergen

Isolation of active peptides from plant hydrolysates that promote Vero cells growth in stirred cultures

Authors: Samia Rourou, Rym Hssiki and Héla Kallel

Comparison of different membrane supports for monolayer culture of bovine oviduct epithelial cells

Authors: Muhammad Z Tahir, Fabien George and Isabelle Donnay

Efficacy of an inactivated and adjuvanted “ZULVAC^®8 OVIS” vaccine produced using single-use bioreactors

Authors: Lídia Garcia, Helena Paradell, Mercedes Mouriño, Berta Alberca, Alicia Urniza, Ana Vila, Margarita Tarrats and Joan Plana-Durán

Engineering CHO cell metabolism for growth in galactose

Authors: Natalia E Jiménez, Camila A Wilkens and Ziomara P Gerdtzen

Engineering CHO cells for improved central carbon and energy metabolism

Authors: Camila A Wilkens and Ziomara P Gerdtzen

Mitogenic effect of sericin on mammalian cells

Authors: Wataru Sato, Ken Fukumoto, Kana Yanagihara, Masahiro Sasaki, Yoshihiro Kunitomi and Satoshi Terada

Challenges in scaling up a perfusion process

Authors: Vana Raja, Saravanan Desan, Ankur Bhatnagar, Anuj Goel and Harish Iyer

High cell density growth of High Five suspension cells in DO-controlled wave-mixed bioreactors

Authors: Teddy Beltrametti, Nicole C Bögli, Gerhard Greller, Regine Eibl and Dieter Eibl

Bag-based rapid and safe seed-train expansion method for Trichoplusia nisuspension cells

Authors: Nicole C Bögli, Christoph Ries, Irina Bauer, Thorsten Adams, Gerhard Greller, Regine Eibl and Dieter Eibl

On-line monitoring of the live cell concentration in bioreactors based on a rocking platform

Authors: John Carvell and Matt Lee

Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments

Authors: Laura Cervera, Sonia Gutiérrez, Francesc Gòdia and María M Segura

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement

Authors: Fabien Dorange, Frédérick Le Goff and Nicolas Dumey

Large pilot scale cultivation process study of adherent MDBK cells for porcine Influenza A virus propagation using a novel disposable stirred-tank bioreactor

Authors: Boris Hundt, Noreen Mölle, Sabine Stefaniak, Ralf Dürrwald and Joerg Weyand

Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells

Authors: Robert Janke, Yvonne Genzel, Maria Wetzel and Udo Reichl

Novel human partner cell line for immortalisation of rare antigen-specific B cells in mAb development

Authors: Galina Kaseko, Marjorie Liu, Qiong Li and Tohsak Mahaworasilpa

Application of the novel and convenient IR/MAR gene amplification technology to the production of recombinant protein pharmaceuticals

Authors: Yoshio Araki, Chiemi Noguchi, Tetsuro Hamafuji, Hiroshi Nose, Daisuke Miki and Noriaki Shimizu

About making a CHO production cell line “research-friendly” by genetic engineering

Authors: Bernd Voedisch, Agnès Patoux, Jildou Sterkenburgh, Mirjam Buchs, Emily Barry, Cyril Allard and Sabine Geisse

CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

Authors: Jens Wölfel, Ruth Essers, Corinna Bialek, Sabine Hertel, Nadine Scholz-Neumann and Gudrun Schiedner

Production and purification of TGFb-1 in CHO-Cells

Authors: Estabraq Abdulkerim, Sabrina Baganz, Axel Schambach, Cornelia Kasper and Thomas Scheper