Key words: protein quantification / cell line development / IgG assay kit / high producer screening / high throughput
The patent pending PAIA technology provides a novel platform for the quantification of proteins, e.g. monoclonal antibodies. PAIA assays consist of functionalized beads to capture the protein of interest (the analyte) and a fluorescent marker to detect the analyte. In contrast to other bead-based assays, which measure the fluorescence intensity of marker that is bound to the beads , PAIA assays measure the fluorescence of unbound marker remaining in solution. We use protrusions on the bottom of the microplate that separate captured analyte-marker complexes from unbound fluorescent marker. Therefore, no washing steps are needed and the assay can be performed in an automation-friendly 384-well plate format (Figure 1A).The time to result, the amount of sample required, and the hands-on time are substantially reduced compared to other immunoassay formats. Hence, this novel approach is particularly suitable for high throughput analysis of protein containing samples like supernatants of antibody producing cells.