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Generation of genetically engineered CHO cell lines to support the production of a difficult to express therapeutic protein

Authors: Holger Laux, Sandrine Romand, Anett Ritter, Mevion Oertli, Mara Fornaro, Thomas Jostock and Burkhard Wilms

Citation: BMC Proceedings 2013 7(Suppl 6):P1

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Multidimension cultivation analysis by standard and omics methods for optimization of therapeutics production

Authors: Julia Gettmann, Christina Timmermann, Jennifer Becker, Tobias Thüte, Oliver Rupp, Heino Büntemeyer, Anica Lohmeier, Alexander Goesmann and Thomas Noll

Citation: BMC Proceedings 2013 7(Suppl 6):P5

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

Authors: Kyoung Ho Lee, Kohsuke Honda, Hisao Ohtake and Takeshi Omasa

Citation: BMC Proceedings 2013 7(Suppl 6):O7

Content type: Oral presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Highly efficient inoculum propagation in perfusion culture using WAVE Bioreactor™ systems

Authors: Christian Kaisermayer and Jianjun Yang

Citation: BMC Proceedings 2013 7(Suppl 6):P7

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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BEAT^® the bispecific challenge: a novel and efficient platform for the expression of bispecific IgGs

Authors: Pierre Moretti, Darko Skegro, Romain Ollier, Paul Wassmann, Christel Aebischer, Thibault Laurent, Miriam Schmid-Printz, Roberto Giovannini, Stanislas Blein and Martin Bertschinger

Citation: BMC Proceedings 2013 7(Suppl 6):O9

Content type: Oral presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Seed train optimization for suspension cell culture

Authors: Tanja Hernández Rodríguez, Ralf Pörtner and Björn Frahm

Citation: BMC Proceedings 2013 7(Suppl 6):P9

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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In vitro safety assessment of nanosilver with improved cell culture systems

Authors: Alina Martirosyan, Madeleine Polet and Yves-Jacques Schneider

Citation: BMC Proceedings 2013 7(Suppl 6):P10

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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A quantitative and mechanistic model for monoclonal antibody glycosylation as a function of nutrient availability during cell culture

Authors: Ioscani Jiménez del Val, Antony Constantinou, Anne Dell, Stuart Haslam, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2013 7(Suppl 6):O10

Content type: Oral presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Novel approaches to render stable producer cell lines viable for the commercial manufacturing of rAAV-based gene therapy vectors

Authors: Verena V Emmerling, Karlheinz Holzmann, Karin Lanz, Stefan Kochanek and Markus Hörer

Citation: BMC Proceedings 2013 7(Suppl 6):P12

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Advanced off-gas measurement using proton transfer reaction mass spectrometry to predict cell culture parameters

Authors: Timo Schmidberger and Robert Huber

Citation: BMC Proceedings 2013 7(Suppl 6):P14

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Powerful expression in Chinese Hamster Ovary cells using bacterial artificial chromosomes: parameters influencing productivity

Authors: Wolfgang Sommeregger, Andreas Gili, Thomas Sterovsky, Emilio Casanova and Renate Kunert

Citation: BMC Proceedings 2013 7(Suppl 6):P25

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Development of a chemically defined cultivation and transfection medium for HEK cell lines

Authors: Sebastian Püngel, T Tim Welsink, Penélope Villegas Soto, Wolfgang Weglöhner, Tim F Beckmann, Ina Eickmeier, Stefan Northoff and Christoph Heinrich

Citation: BMC Proceedings 2013 7(Suppl 6):P27

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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NIR-spectroscopy for bioprocess monitoring & control

Authors: Marko Sandor, Ferdinand Rüdinger, Dörte Solle, Roland Bienert, Christian Grimm, Sven Groß and Thomas Scheper

Citation: BMC Proceedings 2013 7(Suppl 6):P29

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Cellular tools for biosimilar mAb analysis

Authors: Carsten Lindemann, Silke Mayer, Miriam Engel and Petra Schroeder

Citation: BMC Proceedings 2013 7(Suppl 6):P31

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells

Authors: Agustina Gugliotta, Marcos Oggero-Eberhardt, Marina Etcheverrigaray, Ricardo Kratje and Natalia Ceaglio

Citation: BMC Proceedings 2013 7(Suppl 6):P33

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Development and evaluation of a new, specially tailored CHO media platform

Authors: Tim F Beckmann, Christoph Heinrich, Heino Büntemeyer and Stefan Northoff

Citation: BMC Proceedings 2013 7(Suppl 6):P35

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Temperature dependency of immunoglobulin production in novel human partner cell line

Authors: Galina Kaseko, Marjorie Liu, Edwin Hoe, Qiong Li, Mercedes Ballesteros and Tohsak Mahaworasilpa

Citation: BMC Proceedings 2013 7(Suppl 6):P37

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Impact of single-use technology on continuous bioprocessing

Authors: William G Whitford and Brandon L Pence

Citation: BMC Proceedings 2013 7(Suppl 6):P39

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Size-dependent antioxidative activity of platinum nanoparticles

Authors: Hidekazu Nakanishi, Takeki Hamasaki, Tomoya Kinjo, Kiichiro Teruya, Shigeru Kabayama and Sanetaka Shirahata

Citation: BMC Proceedings 2013 7(Suppl 6):P41

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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¹³C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells

Authors: Judith Wahrheit, Averina Nicolae and Elmar Heinzle

Citation: BMC Proceedings 2013 7(Suppl 6):P43

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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The optimization of a rapid low-cost alternative of large-scale medium sterilization

Authors: Dominique T Monteil, Cédric A Bürki, Lucia Baldi, David L Hacker, Maria de Jesus and Florian M Wurm

Citation: BMC Proceedings 2013 7(Suppl 6):P45

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Approaches for automized expansion and differentiation of human MSC in specialized bioreactors

Authors: Anne Neumann, Antonina Lavrentieva, Dominik Egger, Tim Hatlapatka and Cornelia Kasper

Citation: BMC Proceedings 2013 7(Suppl 6):P47

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Scale-up considerations for monoclonal antibody production process: an oxygen transfer flux approach

Authors: Laura Gimenez, Claire Simonet and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P49

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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HEK293 cell culture media study: increasing cell density for different bioprocess applications

Authors: Leticia Liste-Calleja, Martí Lecina and Jordi Joan Cairó

Citation: BMC Proceedings 2013 7(Suppl 6):P51

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Adhesion and colonization of mesenchymal stem cells on polylactide or PLCL fibers dedicated for tissue engineering

Authors: Frédérique Balandras, Caroline Ferrari, Eric Olmos, Mukesh Gupta, Cécile Nouvel, Jérôme Babin, Jean-Luc Six, Nguyen Tran, Isabelle Chevalot, Emmanuel Guedon and Annie Marc

Citation: BMC Proceedings 2013 7(Suppl 6):P53

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Using Rice Bran Extract (RBE) as Supplement for Mescenchymal Stem Cells (MSCs) in Serum-free Culture

Authors: Rinaka Yamauchi, Ken Fukumoto, Satoko Moriyama, Masayuki Taniguchi, Shigeru Moriyama, Takuo Tsuno and Satoshi Terada

Citation: BMC Proceedings 2013 7(Suppl 6):P58

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Characterization and quantitation of fluorescent Gag virus-like particles

Authors: Sonia Gutiérrez-Granados, Laura Cervera, Francesc Gòdia and María Mercedes Segura

Citation: BMC Proceedings 2013 7(Suppl 6):P62

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Effects of perfusion processes under limiting conditions on different Chinese Hamster Ovary cells

Authors: Anica Lohmeier, Tobias Thüte, Stefan Northoff, Jeff Hou, Trent Munro and Thomas Noll

Citation: BMC Proceedings 2013 7(Suppl 6):P64

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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A robust RMCE system based on a CHO-DG44 platform enables efficient evaluation of complex biological drug candidates

Authors: Thomas Rose, Annette Knabe, Rita Berthold, Kristin Höwing, Anne Furthmann, Karsten Winkler and Volker Sandig

Citation: BMC Proceedings 2013 7(Suppl 6):P66

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Chemical chaperone suppresses the antibody aggregation in CHO cell culture

Authors: Masayoshi Onitsuka, Miki Tatsuzawa, Masahiro Noda and Takeshi Omasa

Citation: BMC Proceedings 2013 7(Suppl 6):P68

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Fucoidan extract enhances the anti-cancer activity of chemotherapeutic agents in breast cancer cells

Authors: Sanetaka Shirahata, Zhonguan Zhang, Toshihiro Yoshida, Hiroshi Eto and Kiichiro Teruya

Citation: BMC Proceedings 2013 7(Suppl 6):P70

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Dynamic culture of human liver equivalents inside a micro-bioreactor for long-term substance testing

Authors: Eva-Maria Materne, Ilka Wagner, Caroline Frädrich, Ute Süßbier, Reyk Horland, Silke Hoffmann, Sven Brincker, Alexandra Lorenz, Matthias Gruchow, Frank Sonntag, Udo Klotzbach, Roland Lauster and Uwe Marx

Citation: BMC Proceedings 2013 7(Suppl 6):P72

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Optimized fermentation conditions for improved antibody yield in hybridoma cells

Authors: Martina Stützle, Alina Moll, René Handrick and Katharina Schindowski

Citation: BMC Proceedings 2013 7(Suppl 6):P74

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Feeding strategy optimization in interaction with target seeding density of a fed-batch process for monoclonal antibody production

Authors: Marie-Françoise Clincke, Grégory Mathy, Laura Gimenez, Guillaume Le Révérend, Boris Fessler, Jimmy Stofferis, Bassem Ben Yahia, Nicola Bonsu-Dartnall and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P78

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Characterization of mAb aggregates in a mammalian cell culture production process

Authors: Albert Paul and Friedemann Hesse

Citation: BMC Proceedings 2013 7(Suppl 6):P80

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Introducing a new chemically defined medium and feed for hybridoma cell lines

Authors: Christoph Heinrich, Tim F Beckmann, Sandra Klausing, Stefanie Maimann, Bernd Schröder and Stefan Northoff

Citation: BMC Proceedings 2013 7(Suppl 6):P82

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Golgi engineering of CHO cells by targeted integration of glycosyltransferases leads to the expression of novel Asn-linked oligosaccharide structures at secretory glycoproteins

Authors: Tobias Reinl, Nicolas Grammel, Sebastian Kandzia, Eckart Grabenhorst and Harald S Conradt

Citation: BMC Proceedings 2013 7(Suppl 6):P84

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Technology transfer and scale down model development strategy for biotherapeutics produced in mammalian cells

Authors: Nadine Kochanowski and Laetitia Malphettes

Citation: BMC Proceedings 2013 7(Suppl 6):P86

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Platform process will give platform product - Can we afford it?

Authors: Rohit Diwakar, Sunaina Prabhu, Lavanya C Rao, Janani Kanakaraj, Kriti Shukla, Saravanan Desan, Dinesh Baskar, Ankur Bhatnagar and Anuj Goel

Citation: BMC Proceedings 2013 7(Suppl 6):P88

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Understanding cell behavior in cultivation processes - A metabolic approach

Authors: Jonas Aretz, Tobias Thüte, Sebastian Scholz, Klaudia Kersting, Thomas Noll and Heino Büntemeyer

Citation: BMC Proceedings 2013 7(Suppl 6):P90

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Dynamic profiling of amino acid transport and metabolism in Chinese hamster ovary cell culture

Authors: Sarantos Kyriakopoulos, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2013 7(Suppl 6):P97

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components

Authors: Ryan Boniface, Jeoffrey Schageman, Brian Sanderson, Michael Gillmeister, Angel Varela-Rohena, John Yan, Yolanda Tennico, Shawn Barrett, Robert Setterquist and Stephen Gorfien

Citation: BMC Proceedings 2013 7(Suppl 6):P99

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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How to assess chemically defined media and feeds from 9 suppliers on CHO cells producing mAb

Authors: Aurore Polès-Lahille, Margaux Paillet, Aurélie Da Silva, Nora Kadi, Eric Basque, Flavien Thuet, David Balbuena and Sébastien Ribault

Citation: BMC Proceedings 2013 7(Suppl 6):P100

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Culture supplement extracted from rice bran for better serum-free culture

Authors: Satoshi Terada, Satoko Moriyama, Ken Fukumoto, Yui Okada, Rinaka Yamauchi, Yoko Suzuki, Masayuki Taniguchi, Shigeru Moriyama and Takuo Tsuno

Citation: BMC Proceedings 2013 7(Suppl 6):P104

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Rice bran extract (RBE) as supplement for cell culture

Authors: Satoko Moriyama, Ken Fukumoto, Masayuki Taniguchi, Shigeru Moriyama, Takuo Tsuno and Satoshi Terada

Citation: BMC Proceedings 2013 7(Suppl 6):P106

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Protein folding and glycosylation process are influenced by mild hypothermia in batch culture and by specific growth rate in continuous cultures of CHO cells producing rht-PA

Authors: Mauricio Vergara, Silvana Becerra, Julio Berrios, Juan Reyes, Cristian Acevedo, Ramon Gonzalez, Nelson Osses and Claudia Altamirano

Citation: BMC Proceedings 2013 7(Suppl 6):P108

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

Authors: Alfred M Engel, Harald Sobek, Michael Greif, Sebastian Malik, Marco Thomann, Christine Jung, Dietmar Reusch, Doris Ribitsch, Sabine Zitzenbacher, Christiane Luley, Katharina Schmoelzer, Tibor Czabany, Bernd Nidetzky, Helmut Schwab and Rainer Mueller

Citation: BMC Proceedings 2013 7(Suppl 6):P110

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies

Authors: Bo Liu, Carina Villacres-Barragan, Erika Lattova, Maureen Spearman and Michael Butler

Citation: BMC Proceedings 2013 7(Suppl 6):P112

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Characterization of recombinant IgA producing CHO cell lines by qPCR

Immunoglobulin A (IgA) mediates a key role in mucosal immunity and is a promising novel immunotherapeutic candidate. However, difficulties in obtaining enough material often hamper in vivo explorations. We have p...

Authors: David Reinhart, Wolfgang Sommeregger, Monika Debreczeny, Elisabeth Gludovacz and Renate Kunert

Citation: BMC Proceedings 2013 7(Suppl 6):P114

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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Multicellular tumor spheroids in microcapsules as a novel 3D in vitro model in tumor biology

Authors: Elena Markvicheva, Daria Zaytseva-Zotova, Roman Akasov, Sergey Burov, Isabelle Chevalot and Annie Marc

Citation: BMC Proceedings 2013 7(Suppl 6):P116

Content type: Poster presentation Published on: 4 December 2013

This article is part of a Supplement: Volume 7 Supplement 6
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